Coding

Part:BBa_K3989013

Designed by: Tianyu Xu   Group: iGEM21_UZurich   (2021-10-01)


double plant immune elicitors flg22 and elf18 fused with ClyA for OMV display

In this construct, outer membrane-associated protein ClyA (Part:BBa_K811000) is used to display two plant immune elicitors, elf18 (Part:BBa_K3989023) and flg22 (Part:BBa_K3286138), to the surface of bacterial outer membrane vesicles (OMVs) to trigger plant immune responses. To make elf18 and flg22 better recognized by plant immune receptor FLS2, we put multiple linkers and a 3×FLAG tag between ClyA and two elicitors to minimize the steric hindrance.

Usage and Biology

This construct can be used to enhance the plant immune responses triggered by OMVs, please see: 2021 UZurich

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1204
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1204
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1204
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1204
    Illegal NgoMIV site found at 1198
    Illegal AgeI site found at 1162
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None